LOCUS       40924_21235        3357 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGCAP10 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Oshikawa M, Sugai Y, Usami R, Ohtoko K, Toyama S, Kato S.
  TITLE     Fine Expression Profiling of Full-length Transcripts using a 
            Size-unbiased cDNA Library Prepared with the Vector-capping Method
  JOURNAL   DNA Res. 15 (3), 123-136 (2008)
  PUBMED    18487259
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Kato S.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2007) Contact:Seishi Kato National Rehabilitation 
            Center for Persons with Disabilities, Research Institute, Department
            of Rehabilitation Engineering; 4-1 Namiki, Tokorozawa, Saitama 
            359-8555, Japan
REFERENCE   3  (bases 1 to 3357)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3357)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "DNA Res."; 
            date: "2008"; volume: "15"; issue: "3"; pages: "123-136"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-DEC-2007) Contact:Seishi Kato National Rehabilitation Center for
            Persons with Disabilities, Research Institute, Department of 
            Rehabilitation Engineering; 4-1 Namiki, Tokorozawa, Saitama 
            359-8555, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3357
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        18..347
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     intron          386..482
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     526..542
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        550..568
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    601..606
                     /label=KpnI site for dT tailing
                     /note="KpnI site for dT tailing"
     promoter        complement(622..640)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    complement(642..776)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1074..1662)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1836..2693)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2694..2798)
                     /label=AmpR promoter
     rep_origin      complement(2825..3280)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3298..3314
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttggct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
       61 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
      181 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
      241 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
      301 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcctcgagg
      361 aactgaaaaa ccagaaagtt aactggtaag tttagtcttt ttgtctttta tttcaggtcc
      421 cggatccggt ggtggtgcaa atcaaagaac tgctcctcag tggatgttgc ctttacttct
      481 aggcctgtac ggaagtgtta cttctgctct aaaagctgct cgagtgtaaa acgacggcca
      541 gtacgtattt aatacgactc actataggga atttaaatga attcggccgg ccgatatcct
      601 ggtaccgcgg ccgcggatct ccctttagtg agggttaatt ggatccagac atgataagat
      661 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
      721 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
      781 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttctg
      841 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
      901 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      961 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     1021 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     1081 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     1141 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     1201 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     1261 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     1321 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1381 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     1441 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     1501 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     1561 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     1621 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1681 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1741 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1801 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1861 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1921 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1981 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     2041 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     2101 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     2161 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     2221 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     2281 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     2341 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     2401 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     2461 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     2521 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     2581 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     2641 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2701 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2761 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2821 cctgaaattg taaacgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     2881 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     2941 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     3001 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     3061 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     3121 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     3181 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     3241 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcacacag
     3301 gaaacagcta tgaccatgat cctctagagt cgacctgcag gcatgcttaa ttaaggg
//
